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Enhanced Binding Efficiency of Microcantilever Biosensor for the Detection of Yersinia

2019-09-19

Authors: Liu, XC; Wang, LH; Zhao, JY; Zhu, YF; Yang, JL; Yang, FH

SENSORS

Volume: 19 Issue: 15 Published: AUG 1 2019 Language: English Document type: Article

DOI: 10.3390/s19153326

Abstract:

A novel microcantilever sensorwas batch fabricated for Yersinia detection. The microcantilever surface modification method was optimized by introducing a secondary antibody to increase the number of binding sites. A novel microfluidic platform was designed and fabricated successfully. A 30 mu L solution could fully react with the microcantilever surface. Those routines enhanced the binding efficiency between the target and receptor on the microcantilever. With this novel designed microfluidic platform, the specific adsorption of 10(7) Yersinia on the beam surface with modified F1 antibody was significantly enhanced.

Full Text: https://www.mdpi.com/1424-8220/19/15/3326



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